Off the top of my head, SpongeBob SquarePants, Danny Phantom, Fairly Oddparents, The Big Bang Theory and It’s Always Sunny in Philadelphia all have jokes, if not the main plot point of the episode, that are just mocking the existence of LGBTQ people.
Probably the funniest instance of this is South Park doing something horribly transphobic, realizing it years later, retconning it, then undoing any progress made by doing something else horribly transphobic.
god there’s at least 10 extraordinarily transphobic episodes of South Park and each one is a little bit worse than the one before
It really does get worse. You start with Cartmans mom, where its just a throwaway joke. Still transphobic, but its not the point of the episode and probably not something they thought much about. Then you got Mr to Mrs Garrison, where they make a whole character out of it, but there’s usually more going on in her episodes than just making fun of trans people. And then you get to the recent trans athlete episode, which just has no other jokes and is just 22 minutes of whining about trans athletes.