Nope, they still look young enough to me to separate, and tomatoes can be surprisingly hardy. Even if say you managed to break every single root off, you could place the stem in a jar of water and it’ll sprout new roots in a week or so. One way to easily clone a plant, or skip growing a new plant from seed is to clip and root a sucker.

Just carefully get them out of the pot (my favorite gentle method is putting fingers against the soil around the base of the cluster and then flipping the whole pot, but this tends to be messy), and then just gently massage the root ball to remove the dirt or swish in a bucket of water.

Though before you do that, I’d think about where you’re going to plant the other seedlings. I see as many as 7 individual shoots in some of your pots, and a typical rule of thumb is each tomato wants a 5 gallon bucket of soil to itself. You can get away with with smaller containers (some determinate varieties will do fine, like this Orange Hat micro tomato), but it makes them much more sensitive to changes in moisture and nutrients. Personally, I wouldn’t leave more than two max in the pots you’ve got.

If you don’t have the space to spread out your seedlings, you might want to skip uprooting them and just snip the excess seedlings at the base. Or pull them up, and give the spares to friends!